2014/05/30 by Ajar Nath Yadav, Priyanka Verma, Murugan Kumar +9 · 1 citation
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · Environmental Science · #Genomics and Phylogenetic Studies #Probiotics and Fermented Foods #Microbial Community Ecology and Physiology
paper · pdf · doi:10.1007/s13213-014-0897-9
openalex publication_date 2014/05/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
The diversity of culturable, aerobic and heterotrophic Bacillus and Bacillus -derived genera (BBDG) was investigated in various extreme environments (including thermal springs, cold deserts, mangroves, salt lakes, arid regions, salt pans and acidic soils) of India. Heat treatment followed by enrichment in different media led to a total of 893 bacterial isolates. Amplified ribosomal DNA restriction analysis (ARDRA) using three restriction enzymes Alu I, Msp I and Hae III led to the clustering of these isolates into 12–74 groups for the different sites at 75 % similarity index, adding up to 559 groups. Phylogenetic analysis based on 16S rRNA gene sequencing led to the identification of 392 bacilli, grouped in two families, Bacillaceae (89.03 %) and Paenibacillaceae (10.97 %), and included 13 different genera with 75 distinct species. It was found that among the thirteen genera, nine ( Bacillus, Halobacillus, Lysinibacillus , Oceanobacillus , Pontibacillus, Salinibacillus, Sediminibacillus, Thalassobacillus and Virgibacillus ) belonged to Bacillaceae and four ( Ammoniphilus, Aneurinibacillus, Brevibacillus and Paenibacillus ) belonged to Paenibacillaceae. Novel isolates tolerant to low and high pH and temperature, salt and low moisture were identified. The major outcome of the present investigation was the identification of niche-specific species and also the ubiquitous presence of selected species of BBDG, which illustrate the diversity and pervasive nature of BBDG in extreme environments.