1998/01/01 by Anne Chapman‐Smith, John E. Cronan, Ramón López de Lucio · 3 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Economics, Econometrics and Finance · Social Sciences · #Biotin and Related Studies #Cellular transport and secretion #Click Chemistry and Applications #Employment, Labor, and Gender Studies #Historical and socio-economic studies of Spain and related regions #Regional Development and Innovation
paper · doi:10.1016/s0968-0004(99)01438-3
openalex publication_date 1998/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/04/28
Biotin is a coenzyme essential to all life forms. The vitamin has biological activity only when covalently attached to certain key metabolic enzymes. Most organisms have only one enzyme for attachment of biotin to other proteins and the sequences of these proteins and their substrate proteins are strongly conserved throughout nature. Structures of both the biotin ligase and the biotin carrier protein domain from Escherichia coli have been determined. These, together with mutational analyses of biotinylated proteins, are beginning to elucidate the exceptional specificity of this protein modification.