2021/01/01 by Satoshi Morito, Takao Nitanda, Ryuko Tsukamoto +4 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Biomedical engineering #Cancer #Cancer Cells and Metastasis #Cancer Genomics and Diagnostics #Cytology #Cytopathology #Immunocytochemistry #Liquid-based cytology #Lung Cancer Treatments and Mutations #Medicine #Pathology #Radiology
paper · pdf · doi:10.1159/000516555
crossref issued 2021/01/01 · crossref published 2021/01/01 · crossref published-print 2021/01/01 · openalex publication_date 2021/01/01 · crossref published-online 2021/06/07 · crossref created 2021/06/07 · crossref deposited 2025/04/23 · openalex created_date 2025/10/10 · crossref indexed 2026/07/31 · openalex updated_date 2026/08/01
Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3-40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.