2025/01/01 by Scherer, Gerhard, Gilch, Gerhard, Aust, Beate +4
Agricultural and Biological Sciences · Environmental Science · Medicine · #543 Analytical chemistry #543 Analytische Chemie #571.95 Toxicology #571.95 Toxikologie #616.9803 Betriebs- und Arbeitsmedizin #616.9803 Industrial and occupational medicine #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Biomonitoring #Biomonitoringmethode #DMF #Dimethylformamid #Dimethylformamide #Formamid #Formamide #GC-MS #Gas chromatography with mass spectrometry #Gaschromatographie mit Massenspektrometrie #HBM #Hintergrundbelastung #Humanbiomonitoring #Indoor Air Quality and Microbial Exposure #Intoxikation #Methode #N #N-Dimethylformamid #N-Dimethylformamide #Occupational exposure and asthma #Pesticide Residue Analysis and Safety #Umweltbelastung #Urin #Vergiftung #analytical method #background exposure #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #employee #environmental exposure #general population #human biomonitoring #intoxication #method #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi7512d0022_w
openalex publication_date 2025/01/01 · openalex created_date 2025/12/13 · openalex updated_date 2026/07/22
The working group „Analyses in Biological Materials“ of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method. The method described herein allows the determination of formamide in urine by gas chromatography mass spectrometry (GC‐MS). Due to its sensitivity, this method is suitable for the detection of occupational and environmental exposure to formamide. For the analytical determination 1 ml of urine is lyophilized after being spiked with 13C,15N‐formamide as the internal standard. The lyophilisate is extracted with 200 µl methanol. After centrifugation, 1 µl of the extract is injected into a GC‐MS system. The method was extensively validated and the reliability data were confirmed by an independent laboratory, which has established and cross‐checked the whole procedure.