The central scaffold protein CEP350 coordinates centriole length, stability, and maturation

2022/10/31 by Onur Rojhat Karasu, Annett Neuner, Enrico Salvatore Atorino +2 · 7 citations
Biochemistry, Genetics and Molecular Biology · #Microtubule and mitosis dynamics #Photosynthetic Processes and Mechanisms #Protist diversity and phylogeny

paper · doi:10.1083/jcb.202203081

Abstract

The centriole is the microtubule-based backbone that ensures integrity, function, and cell cycle-dependent duplication of centrosomes. Mostly unclear mechanisms control structural integrity of centrioles. Here, we show that the centrosome protein CEP350 functions as scaffold that coordinates distal-end properties of centrioles such as length, stability, and formation of distal and subdistal appendages. CEP350 fulfills these diverse functions by ensuring centriolar localization of WDR90, recruiting the proteins CEP78 and OFD1 to the distal end of centrioles and promoting the assembly of subdistal appendages that have a role in removing the daughter-specific protein Centrobin. The CEP350-FOP complex in association with CEP78 or OFD1 controls centriole microtubule length. Centrobin safeguards centriole distal end stability, especially in the compromised CEP350-/- cells, while the CEP350-FOP-WDR90 axis secures centriole integrity. This study identifies CEP350 as a guardian of the distal-end region of centrioles without having an impact on the proximal PCM part.

Citations

• Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis

Cited by

• Interactome Analysis Reveals a Link of the Novel ALMS1-CEP70 Complex to Centrosomal Clusters. [europepmc]
• Predicting potential target genes in molecular biology experiments using machine learning and multifaceted data sources. [europepmc]
• Spermatocytes have the capacity to segregate chromosomes despite centriole duplication failure. [europepmc]
• A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function. [europepmc]
• Meiotic divisions and round spermatid formation do not require centriole duplication in mice. [europepmc]
• Mechanisms underlying centriole stability. [europepmc]
• A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function [europepmc]

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