The phosphoprotein stathmin is essential for nerve growth factor-stimulated differentiation.

1996/06/15 by Gilbert Di Paolo, G Di Paolo, V Pellier +8 · 5 citations
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Cellular Mechanics and Interactions #Microtubule and mitosis dynamics #Axon Guidance and Neuronal Signaling

paper · pdf · doi:10.1083/jcb.133.6.1383

Abstract

Stathmin is a ubiquitous cytosolic protein which undergoes extensive phosphorylation in response to a variety of external signals. It is highly abundant in developing neurons. The use of antisense oligonucleotides which selectively block stathmin expression has allowed us to study directly its role in rat PC12 cells. We show that stathmin depletion prevents nerve growth factor (NGF)-stimulated differentiation of PC12 cells into sympathetic-like neurons although the expression of several NGF-inducible genes was not affected. Furthermore, we found that stathmin phosphorylation in PC12 cells which is induced by NGF depends on mitogen-activated protein kinase (MAPK) activity. We conclude that stathmin is an essential component of the NGF-induced MAPK signaling pathway and performs a key role during differentiation of developing neurons.

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• Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10. [europepmc]
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