2002/02/11 by Frédéric Mallard, Bor Luen Tang, Thierry Galli +7 · 8 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Cellular transport and secretion #Lysosomal Storage Disorders Research #Pancreatic function and diabetes
paper · pdf · doi:10.1083/jcb.200110081
openalex publication_date 2002/02/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
The molecular mechanisms underlying early/recycling endosomes-to-TGN transport are still not understood. We identified interactions between the TGN-localized putative t-SNAREs syntaxin 6, syntaxin 16, and Vti1a, and two early/recycling endosomal v-SNAREs, VAMP3/cellubrevin, and VAMP4. Using a novel permeabilized cell system, these proteins were functionally implicated in the post-Golgi retrograde transport step. The function of Rab6a' was also required, whereas its closely related isoform, Rab6a, has previously been implicated in Golgi-to-endoplasmic reticulum transport. Thus, our study shows that membrane exchange between the early endocytic and the biosynthetic/secretory pathways involves specific components of the Rab and SNARE machinery, and suggests that retrograde transport between early/recycling endosomes and the endoplasmic reticulum is critically dependent on the sequential action of two members of the Rab6 subfamily.