Fluorescence visualization of cellulose and pectin in the primary plant cell wall

2020/04/09 by Amir J. Bidhendi, Youssef Chebli, Anja Geitmann · 2 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Polysaccharides and Plant Cell Walls #Plant Reproductive Biology #Plant Molecular Biology Research

paper · doi:10.1111/jmi.12895

openalex publication_date 2020/04/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/11

Abstract

Plant cell walls constitute the extracellular matrix surrounding plant cells and are composed mainly of polysaccharides. The chemical makeup of the primary plant cell wall, and specifically, the abundance, localization and arrangement of the constituting polysaccharides are intimately linked with growth, morphogenesis and differentiation in plant cells. Visualization of the cell wall components is, therefore, a crucial tool in plant cell developmental studies. In this technical update, we present protocols for fluorescence visualization of cellulose and pectin in selected plant tissues and illustrate examples of some of the available labels that hold promise for live imaging of plant cell wall expansion and morphogenesis.

Citations

• A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
• Relating the mechanics of the primary plant cell wall to morphogenesis
• Synergistic Pectin Degradation and Guard Cell Pressurization Underlie Stomatal Pore Formation

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• Physical, mechanical, and microstructural characterization of novel, 3D-printed, tunable, lab-grown plant materials generated from Zinnia elegans cell cultures
• Cellulose [wikipedia]

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