2003/06/19 by Sandra Stegemann, Stefanie Hartmann, Stephanie Ruf +1 · 5 citations
Biochemistry, Genetics and Molecular Biology · #Photosynthetic Processes and Mechanisms #Genomics and Phylogenetic Studies #Plant tissue culture and regeneration
paper · doi:10.1073/pnas.1430924100
openalex publication_date 2003/06/19 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
Eukaryotic cells arose through endosymbiotic uptake of free-living bacteria followed by massive gene transfer from the genome of the endosymbiont to the host nuclear genome. Because this gene transfer took place over a time scale of hundreds of millions of years, direct observation and analysis of primary transfer events has remained difficult. Hence, very little is known about the evolutionary frequency of gene transfer events, the size of transferred genome fragments, the molecular mechanisms of the transfer process, or the environmental conditions favoring its occurrence. We describe here a genetic system based on transgenic chloroplasts carrying a nuclear selectable marker gene that allows the efficient selection of plants with a nuclear genome that carries pieces transferred from the chloroplast genome. We can select such gene transfer events from a surprisingly small population of plant cells, indicating that the escape of genetic material from the chloroplast to the nuclear genome occurs much more frequently than generally believed and thus may contribute significantly to intraspecific and intraorganismic genetic variation.