The ErpA/NfuA complex builds an oxidation-resistant Fe-S cluster delivery pathway

2018/04/06 by Béatrice Py, Catherine Gerez, Allison Huguenot +4 · 23 citations
Energy · Nursing · Biochemistry, Genetics and Molecular Biology · #Metalloenzymes and iron-sulfur proteins #Trace Elements in Health #Porphyrin Metabolism and Disorders

paper · pdf · doi:10.1074/jbc.ra118.002160

Abstract

= 90 min. Using genetic and plasmon surface resonance analyses, we showed that NfuA and ErpA interacted directly with client proteins, whereas IscA or SufA did not. Moreover, NfuA and ErpA interacted with one another. Given all of these observations, we propose an architecture of the Fe-S delivery network in which ErpA is the last factor that delivers cluster directly to most if not all client proteins. NfuA is proposed to assist ErpA under severely unfavorable conditions. A comparison with the strategy employed in yeast and eukaryotes is discussed.

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