2013/09/06 by Tina Schrödel, Robert Prevedel, Karin Aumayr +2 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · Neuroscience · Physics and Astronomy · #Circadian rhythm and melatonin #Genetics, Aging, and Longevity in Model Organisms #Spaceflight effects on biology #physics.bio-ph #physics.optics #q-bio.NC
paper · pdf · doi:10.1038/nmeth.2637
published as Nature Methods 10, 1013-1020 (2013) · 28 pages, 5 figures, plus Supplementary Information (24 pages, 10 figures)
openalex publication_date 2013/09/06 · arxiv created 2014/06/06 · arxiv updated 2014/06/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Recent efforts in neuroscience research seek to obtain detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of C. elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans using wide-field temporal focusing (WF-TEFO). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator (NLS-GCaMP5K) that permits unambiguous discrimination of individual neurons within the densely-packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a lab-on-a-chip device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.