Cyclic AMP (cAMP) and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 inEscherichia coli
2005/03/02 by Liang Wang, Yoshifumi Hashimoto, Chen-Yu Tsao +4 · 194 citations
Biochemistry, Genetics and Molecular Biology · #Autoinducer #Bacterial Genetics and Biotechnology #Bacterial biofilms and quorum sensing #Biochemistry #Biology #Catabolite repression #Cell biology #Escherichia coli #Extracellular #Gene #Gene expression #Microbiology #Operon #Promoter #Quorum sensing #Repressor #Salmonella enterica #Transcription factor #Vibrio bacteria research studies #Virulence #cAMP receptor protein
paper · open access · doi:10.1128/jb.187.6.2066-2076.2005
published in Journal of Bacteriology 187(6), 2066-2076 (American Society for Microbiology)
openalex publication_date 2005/03/02 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/22
Abstract
Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes.
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