AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants

1996/01/01 by H Okusu, D Ma, Hiroshi Nikaido +1 · 27 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Antibiotic Resistance in Bacteria #Cancer therapeutics and mechanisms #Antibiotics Pharmacokinetics and Efficacy

paper · doi:10.1128/jb.178.1.306-308.1996

Abstract

Multiple-antibiotic-resistance (Mar) mutants of Escherichia coli are resistant to a wide variety of antibiotics, and increased active efflux is known to be responsible for the resistance to some drugs. The identity of the efflux system, however, has remained unknown. By constructing an isogenic set of E. coli K-12 strains, we showed that the marR1 mutation was incapable of increasing the resistance level in the absence of the AcrAB efflux system. This experiment identified the AcrAB system as the major pump responsible for making the Mar mutants resistant to many agents, including tetracycline, chloramphenicol, ampicillin, nalidixic acid, and rifampin.

Citations

• Prevention of Drug Access to Bacterial Targets: Permeability Barriers and Active Efflux

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