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CotA of <i>Bacillus subtilis</i> Is a Copper-Dependent Laccase

2001/09/15 by Marie-Françoise Hullo, Marie-Françoise Hullo, Ivan Moszer +3 · 30 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Enzyme-mediated dye degradation #Amino Acid Enzymes and Metabolism #Microbial Metabolism and Applications

paper · doi:10.1128/jb.183.18.5426-5430.2001

Abstract

The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA nor other sporulation proteins are involved. We demonstrate that CotA is a laccase. Syringaldazine, a specific substrate of laccases, reacted with wild-type spores but not with DeltacotA spores. CotA may participate in the biosynthesis of the brown spore pigment, which appears to be a melanin-like product and to protect against UV light.

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