Phosphosugar Stress in Bacillus subtilis: Intracellular Accumulation of Mannose 6-Phosphate Derepressed the glcR-phoC Operon from Repression by GlcR

2019/02/20 by Kambiz Morabbi Heravi, Irfan Manzoor, Hildegard Watzlawick +3 · 5 citations
Biochemistry, Genetics and Molecular Biology · Materials Science · #Bacterial Genetics and Biotechnology #Enzyme Structure and Function #Protein Structure and Dynamics

paper · pdf · doi:10.1128/jb.00732-18

Abstract

Bacillus subtilis has different stress response systems to cope with external and internal stressors. Here, we investigated how B. subtilis deals with the high intracellular concentration of phosphosugars as an internal stressor. The results indicated the derepression of an operon consisting of a repressor (GlcR) and a phosphatase (PhoC). Further analysis revealed that this operon is not a phosphosugar stress response system. The substrate specificity of PhoC may indicate a connection between the glcR-phoC operon and pathways in which glycerol 3-phosphate and ribose 5-phosphate are utilized, such as membrane biosynthesis and teichoic acid elongation.

Cited by

• Sugar-Phosphate Metabolism Regulates Stationary-Phase Entry and Stalk Elongation in Caulobacter crescentus. [europepmc]
• Expression and purification of the 5'-nucleotidase YitU from Bacillus species: its enzymatic properties and possible applications in biotechnology. [europepmc]
• Sugar-Phosphate Toxicities. [europepmc]
• Lumen and mucosa-associated Lactobacillus rhamnosus from the intestinal tract of organ donors. [europepmc]
• Often in silico , rarely in vivo : characterizing endemic plant-associated microbes for system-appropriate biofertilizers. [europepmc]

Related

• (p)ppGpp: Still Magical?
• Evolution of signalling in the sporulation phosphorelay
• Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A
• Autoregulation of the Master Regulator Spo0A Controls Cell‐Fate Decisions in Bacillus subtilis
• Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex