On electron imaging of small molecules, Part II: tomographic reconstruction from defocus series

2019/11/25 by Timur E. Gureyev, T. E. Gureyev, Harry M. Quiney +9
Biochemistry, Genetics and Molecular Biology · Engineering · Materials Science · Physics and Astronomy · #Advanced Electron Microscopy Techniques and Applications #Advanced X-ray Imaging Techniques #Electron and X-Ray Spectroscopy Techniques #FOS: Electrical engineering #FOS: Physical sciences #Image and Video Processing (eess.IV) #Optics (physics.optics) #eess.IV #electronic engineering #information engineering #physics.optics

paper · pdf · doi:10.48550/arxiv.1911.11598

19 pages, 5 figures

openalex publication_date 2019/11/25 · arxiv created 2019/11/28 · arxiv updated 2019/12/02 · openalex created_date 2019/12/05 · openalex updated_date 2026/07/28

Abstract

A practical method utilising three-dimensional image pattern matching is proposed which, in principle, is capable of unambiguous determination of the types and positions of atoms in small molecules from defocus series collected at only a few different angular orientations of the molecule. Numerical tests of the method are presented using multislice calculations of defocus series of small biological molecules. The proposed technique can in future gain from Bayesian or machine-learning approaches and is likely to be useful in cryogenic electron microscopy.

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