2017/10/19 by Didier Y. R. Stainier, Erez Raz, Nathan D. Lawson +16 · 2 citations
Biochemistry, Genetics and Molecular Biology · #Zebrafish Biomedical Research Applications #Developmental Biology and Gene Regulation #Congenital heart defects research
paper · pdf · doi:10.1371/journal.pgen.1007000
openalex publication_date 2017/10/19 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
The zebrafish (Danio rerio) has emerged as a powerful model to study vertebrate development and disease. Its short generation time makes it amenable to genetic manipulation and analysis, and its small size and high fecundity make it especially well suited for large-scale forward genetic and chemical screens. Fast-developing zebrafish embryos are transparent, facilitating live imaging of a variety of developmental processes in wild-type and mutant animals. ...\nThis brief document provides an updated set of guidelines regarding morpholino use in zebrafish that we anticipate will be of value for experimentalists as well as journal and grant reviewers, and decision makers.