1998/05/08 by James E. Ferrell, Eric M. Machleder · 13 citations
Medicine · Neuroscience · Biochemistry, Genetics and Molecular Biology · #Reproductive Biology and Fertility #Circadian rhythm and melatonin #Genetics, Aging, and Longevity in Model Organisms
paper · doi:10.1126/science.280.5365.895
openalex publication_date 1998/05/08 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/31
Xenopus oocytes convert a continuously variable stimulus, the concentration of the maturation-inducing hormone progesterone, into an all-or-none biological response-oocyte maturation. Here evidence is presented that the all-or-none character of the response is generated by the mitogen-activated protein kinase (MAPK) cascade. Analysis of individual oocytes showed that the response of MAPK to progesterone or Mos was equivalent to that of a cooperative enzyme with a Hill coefficient of at least 35, more than 10 times the Hill coefficient for the binding of oxygen to hemoglobin. The response can be accounted for by the intrinsic ultrasensitivity of the oocyte's MAPK cascade and a positive feedback loop in which the cascade is embedded. These findings provide a biochemical rationale for the all-or-none character of this cell fate switch.