2009/08/04 by CBOL Plant Working Group1, Peter M. Hollingsworth, Laura L. Forrest +50 · 5 citations
Biochemistry, Genetics and Molecular Biology · #Genomics and Phylogenetic Studies #Identification and Quantification in Food #Genetic diversity and population structure
paper · doi:10.1073/pnas.0905845106
openalex publication_date 2009/08/04 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/03
DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.