2008/01/01 by Himanshu Vashistha, Mohammad Husain, Dileep Kumar +3 · 1 citation
Immunology and Microbiology · Medicine · #HIV Research and Treatment #HIV/AIDS drug development and treatment #Renal Diseases and Glomerulopathies
paper · doi:10.1080/08860220802134672
openalex publication_date 2008/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Human renal biopsy studies suggest the presence of HIV-1 and associated signs of injury in renal tubular epithelial cells. Because renal epithelial cells lack conventional HIV-1 receptors, the modus operandi of HIV-1 in the induction of tubular cell injury remains a mystery. In the present study, we evaluated the role of HIV-1 gene expression in human proximal tubular cell apoptosis and cell cycle progression. HIV-1- or vector-transduced cells were assayed for cellular injury and cell cycle defect. HIV-1-transduced cells showed the progressive loss of viability in a time-dependent manner. Similarly, HIV-1-transduced cells showed greater apoptosis when compared with vector-transduced cells. A higher number of HIV-1 expressing cells showed cell cycle arrest at G2/M phase and enhanced tubular cell expression of phospho-p53(ser15), phospho-cdc-2(Tyr 15), and phospho-chk-2 (Thr 68). These findings suggest that in addition to the activation of apoptotic pathway, HIV-1-induced G2/M arrest may also contribute to tubular cell injury.