Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers

1997/01/03 by P. Calvo, Pilar Calvo, C. Remu��n-L�pez +3 · 1,850 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Materials Science · Pharmacology, Toxicology and Pharmaceutics · #Advanced Drug Delivery Systems #Aqueous solution #Bovine serum albumin #Chemical engineering #Chemistry #Chitosan #Chromatography #Copolymer #Ethylene oxide #Ionic strength #Materials science #Nanoparticle #Nanotechnology #Organic chemistry #Polyethylene #Polymer #Polymer Surface Interaction Studies #Polymer chemistry #Propylene oxide #Protein purification and stability #Zeta potential

paper · doi:10.1002/(sici)1097-4628(19970103)63:1<125::aid-app13>3.0.co;2-4

published in Journal of Applied Polymer Science 63(1), 125-132 (Wiley)

openalex publication_date 1997/01/03 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05

Abstract

Hydrophilic nanoparticulate carriers have important potential applications for the administration of therapeutic molecules. The recently developed hydrophobic-hydrophilic carriers require the use of organic solvents for their preparation and have a limited protein-loading capacity. To address these limitations a new approach for the preparation of nanoparticles made solely of hydrophilic polymers is presented. The preparation technique, based on an ionic gelation process, is extremely mild and involves the mixture of two aqueous phases at room temperature. One phase contains the polysaccharide chitosan (CS) and a diblock copolymer of ethylene oxide and propylene oxide (PEO-PPO) and, the other, contains the polyanion sodium tripolyphosphate (TPP). Size (200–1000 nm) and zeta potential (between +20 mV and +60 mV) of nanoparticles can be conveniently modulated by varying the ratio CS/PEO-PPO. Furthermore, using bovine serum albumin (BSA) as a model protein it was shown that these new nanoparticles have a great protein loading capacity (entrapment efficiency up to 80% of the protein) and provide a continuous release of the entrapped protein for up to 1 week. © 1997 John Wiley & Sons, Inc.

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