2019/02/04 by Doaa Ghaith, Zeinat K. Mohamed, Mohamed G. Farahat +2 · 2 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Antibiotic Resistance in Bacteria #Biology #Carbapenem-resistant enterobacteriaceae #Colonization #Enterobacteriaceae #Enterobacteriaceae and Cronobacter Research #Escherichia coli #Escherichia coli research studies #Gene #Infection control #Intensive care #Intensive care medicine #Klebsiella oxytoca #Klebsiella pneumoniae #Medicine #Microbiology
paper · pdf · doi:10.1016/j.ajg.2019.01.002
openalex publication_date 2019/02/04 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
Background and study aims: Colonized patients with carbapenamase producing Enterobacteriaceae (CPE) are vulnerable to invasive infections from their endogenous flora. We aimed to assess faecal colonization with (CPE) among children admitted to Cairo University paediatric intensive care units (ICUs). The phenotypic and genotypic characterizations of carbapenemase-producing Enterobacteriaceae were also studied. Patients and methods: A total of 413 Enterobacteriaceae isolates have been isolated from cultured rectal swabs of 100 children. All swabs were inoculated on ChromID TM CARBA agar to screen for carbapenem resistant Enterobacteriaceae (CRE). Disk diffusion method, Modified Hodge test (MHT) and further genotypic detection of carbapenemases genes (bla OXA-48 , bla KPC and bla NDM-1 , bla VIM and bla IMP ) by multiplex PCR were done. Results: Out of 413 Enterobacteriaceae isolates; 100 isolates were defined as CRE. Bla OXA-48 was detected in (33%); Escherichia coli (n = 11), Klebsiella oxytoca (n = 3) and Klebsiella pneumoniae (n = 19), while (27%) carried bla NDM-1 Escherichia coli (n = 7), and Klebsiella pneumoniae (n = 20). Conclusion: Prevalence of carbapenem resistant Enterobacteriaceae was 24%, various genes of carbapenemases were detected in 80% of carbapenem resistant Enterobacteriaceae with dominance of bla OXA-48 . Understanding the colonization status of our patients with strict infection control measures can reduce the risk of horizontal gene transfer of carbapenemases.