1989/01/13 by Steven B. Smith, Paul K. Aldridge, James B. Callis · 4 citations
Engineering · Chemical Engineering · #Microfluidic and Capillary Electrophoresis Applications #Microfluidic and Bio-sensing Technologies #Rheology and Fluid Dynamics Studies
paper · doi:10.1126/science.2911733
openalex publication_date 1989/01/13 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Individual DNA molecules undergoing agarose gel electrophoresis were viewed with the aid of a fluorescence microscope. Molecular shape and orientation were studied in both steady and pulsed electric fields. It was observed that (i) DNA macromolecules advanced lengthwise through the gel in an extended configuration, (ii) the molecules alternately contracted and lengthened as they moved, (iii) the molecules often became hooked around obstacles in a U-shape for extended periods, and (iv) the molecules displayed elasticity as they extended from both ends at once. A computer model has been developed that simulates the migration of the molecules in a rotating-field gel electrophoresis experiment.