2012/01/20 by Jean-Nicolas Longchamp, Tatiana Latychevskaia, Conrad Escher +2
Biochemistry, Genetics and Molecular Biology · Chemistry · Physics and Astronomy · #Advanced Electron Microscopy Techniques and Applications #Advanced X-ray Imaging Techniques #Biochemistry #Biology #Biomolecule #Biophysics #Chemical physics #Chemistry #Cluster (spacecraft) #Computer science #Crystallography #Crystallography and Radiation Phenomena #Ferritin #Holography #Materials science #Molecular biophysics #Molecule #Nanotechnology #Nuclear magnetic resonance #Optics #Physics #Protein structure #Resolution (logic) #Structural biology #physics.bio-ph
paper · pdf · doi:10.1063/1.4748113
published as Appl. Phys. Lett. 101, 093701 (2012)
arxiv created 2012/01/20 · openalex publication_date 2012/08/27 · arxiv updated 2012/09/27 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
Imaging a single biomolecule at atomic resolution without averaging over different conformations is the ultimate goal in structural biology. We report recordings of a protein at nanometer resolution obtained from one individual ferritin by means of low-energy electron holography. One single protein could be imaged for an extended period of time without any sign of radiation damage. Since the fragile protein shell encloses a robust iron cluster, the holographic reconstructions could also be cross-validated against transmission electron microscopy images of the very same molecule by imaging its iron core.