Automated detection of spontaneous calcium signaling events in prohemocytes of the Drosophila melanogaster lymph gland

2025/11/15

paper · doi:10.17912/micropub.biology.001883

Abstract

<p>Calcium signaling is an important regulator of stem cell maintenance and differentiation. Here we report the development of an image processing pipeline for <i>ex vivo</i> time-lapse microscopy data that enables the unbiased, automated detection of calcium signaling events in prohemocytes of the <i>Drosophila melanogaster </i>lymph gland. We also show that heterogeneity in gene expression driven by Tep4-Gal4, which is used to mark prohemocytes, accounts for most of the cell-to-cell variability in the signal, and that spontaneous calcium signaling events in the lymph gland can last from a few seconds to well over a minute.</p>

Related

• DeXtrusion: automatic recognition of epithelial cell extrusion through machine learning in vivo
• A New Registration Approach for Dynamic Analysis of Calcium Signals in Organs
• Mapping the spatiotemporal dynamics of calcium signaling in cellular neural networks using optical flow
• A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging
• Investigating the origin of spontaneous cytosolic calcium oscillations in stomatal guard cells: a dialogue between apoplastic and endoplasmic reticulum stores