1995/07/03 by Hyunsoo Han, M. Michael Wolfe, S. Brenner +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Chemical Synthesis and Analysis #Monoclonal and Polyclonal Antibodies Research #Biochemical and Structural Characterization
paper · doi:10.1073/pnas.92.14.6419
openalex publication_date 1995/07/03 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/15
A concept termed liquid-phase combinatorial synthesis (LPCS) is described. The central feature of this methodology is that it combines the advantages that classic organic synthesis in solution offers with those that solid-phase synthesis can provide, through the application of a linear homogeneous polymer. To validate this concept two libraries were prepared, one of peptide and the second of nonpeptide origin. The peptide-based library was synthesized by a recursive deconvolution strategy [Erb, E., Janda, K. D. & Brenner, S. (1994) Proc. Natl. Acad. Sci. USA 91, 11422-11426] and several ligands were found within this library to bind a monoclonal antibody elicited against beta-endorphin. The non-peptide molecules synthesized were arylsulfonamides, a class of compounds of known clinical bactericidal efficacy. The results indicate that the reaction scope of LPCS should be general, and its value to multiple, high-throughput screening assays could be of particular merit, since multimilligram quantities of each library member can readily be attained.