2013/09/20 by Selin Kara, Dominik Spickermann, Joerg H. Schrittwieser +4 · 1 citation
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Enzyme Catalysis and Immobilization #Enzyme-mediated dye degradation #Microbial Metabolic Engineering and Bioproduction
paper · doi:10.1021/cs400535c
openalex publication_date 2013/09/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/20
The oxidative lactonization of 1,4-, 1,5-, and 1,6-diols using horse liver alcohol dehydrogenase (HLADH) is reported. Molecular oxygen was used as terminal electron acceptor by utilization of the laccase-mediator concept to regenerate the oxidized nicotinamide cofactor and producing water as sole byproduct. Spontaneous hydrolysis of the lactone products was identified as a major limiting factor toward preparative application of the system, which can be alleviated by using a two liquid phase approach to extracting the product into an organic solvent.