2022/02/01 by Shengtao Gao, Alateng Zhula, Wenhui Liu +6 · 6 citations
Agricultural and Biological Sciences · Chemistry · Veterinary · #Animal health and immunology #Barrier function #Biochemistry #Biology #Cell biology #Chemistry #Endocrinology #Epithelium #Ex vivo #Fermentation #Histamine #In vitro #Lipopolysaccharide #Reproductive Physiology in Livestock #Rumen #Ruminant Nutrition and Digestive Physiology #Tight junction #Ussing chamber
paper · doi:10.1093/jas/skac005
openalex publication_date 2022/02/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/25
Disruption of the ruminal epithelium barrier occurs during subacute ruminal acidosis due to low pH, hyper-osmolality, and increased concentrations of lipopolysaccharide and histamine in ruminal fluid. However, the individual roles of lipopolysaccharide and histamine in the process of ruminal epithelium barriers disruption are not clear. The objective of the present investigation was to evaluate the direct effect of lipopolysaccharide and histamine on the barrier function of the ruminal epithelium. Compared with control (CON), histamine (HIS, 20 μM) increased the short-circuit current (Isc; 88.2%, P < 0.01), transepithelial conductance (Gt; 29.7%, P = 0.056), and the permeability of fluorescein 5(6)-isothiocyanate (FITC) (1.04-fold, P < 0.01) of ruminal epithelium. The apparent permeability of LPS was 1.81-fold higher than HIS (P < 0.01). The mRNA abundance of OCLN in ruminal epithelium was decreased by HIS (1.1-fold, P = 0.047). The results of the present study suggested that mucosal histamine plays a direct role in the disruption of ruminal epithelium barrier function, whereas lipopolysaccharide (at a pH of 7.4) has no effect on the permeability of rumen tissues ex vivo.