2005/02/14 by Mark Bates, Timothy R. Blosser, Xiaowei Zhuang · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · Neuroscience · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Molecular Junctions and Nanostructures #Photoreceptor and optogenetics research #physics.bio-ph #physics.chem-ph #q-bio.BM #q-bio.QM
paper · pdf · doi:10.1103/physrevlett.94.108101
Article contains 4 pages and 4 figures. Accepted for publication in Physical Review Letters. Supplementary material and movie file will be available through the PRL web site at the time of publication
arxiv created 2005/02/14 · openalex publication_date 2005/03/15 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore (Cy5) that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore (Cy3) that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of conventional Fo rster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level.