2003/10/27 by C. Danilowicz, Claudia Danilowicz, Y. Kafri +8 · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · Environmental Science · Physics and Astronomy · #Bacteriophages and microbial interactions #DNA and Nucleic Acid Chemistry #Nanopore and Nanochannel Transport Studies #cond-mat.soft #q-bio.BM
paper · pdf · doi:10.1103/physrevlett.93.078101
17 pages, 2 figures
arxiv created 2003/10/27 · openalex publication_date 2004/08/10 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We separate double stranded lambda phage DNA by applying a fixed force at a constant temperature ranging from 15 to 50 degrees C, and measure the minimum force required to separate the two strands. The measurements also offer information on the free energy of double stranded DNA (dsDNA) at temperatures where dsDNA does not thermally denature in the absence of force. While parts of the phase diagram can be explained using existing models and free energy parameters, others deviate significantly. Possible reasons for the deviations between theory and experiment are considered.