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Colorimetric Determination of Aconitine in Galenicals and in Biological Samples

1976/07/01 by Mohamed S Karawya, Abdel-Aziz A Sharaf, Aly M Diab · 1 citation

paper · doi:10.1093/jaoac/59.4.799

crossref issued 1976/07/01 · crossref published 1976/07/01 · crossref published-print 1976/07/01 · crossref published-online 2020/01/29 · crossref created 2020/02/17 · crossref deposited 2020/03/19 · crossref indexed 2026/08/01

Abstract

Abstract A sensitive method is presented for determining aconitine. Aconitine is complexed with Co2+, the aconitine-cobalt complex is extracted with chloroform, and the absorbance is measured at 320 nm. The sensitivity of the method ranged between 0.06 and 3 mg/25 ml, and the color was stable for 6 hr. The method was successfully applied for the quantitative determination of aconitine in animal tissues.

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