2015/10/01 by Gražvydas Lukinavičius, Claudia Blaukopf, Elias Pershagen +8 · 2 citations
Biochemistry, Genetics and Molecular Biology · #Advanced Fluorescence Microscopy Techniques #Cell Image Analysis Techniques #Molecular Biology Techniques and Applications
paper · pdf · doi:10.1038/ncomms9497
openalex publication_date 2015/10/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.