Phylogenetic analysis of spotted fever group rickettsiae by study of the outer surface protein rOmpA
1998/07/01 by P.-E. Fournier, Pierre‐Edouard Fournier, V. Roux +3 · 29 citations
Immunology and Microbiology · Agricultural and Biological Sciences · #Vector-borne infectious diseases #Insect symbiosis and bacterial influences #Plant Parasitism and Resistance
paper · doi:10.1099/00207713-48-3-839
Abstract
Rickettsiae are classified in the order Rickettsiales and have been included in the alpha subclass of the class Proteobacteria on the basis of 16S rRNA gene sequence comparison. To estimate the evolutionary forces that have shaped the members of the spotted fever group (SFG) rickettsiae, the ompA gene (apart from the tandem repeat units), encoding an antigenic high-molecular-mass membrane protein specific for the group, was amplified and sequenced from 21 isolates. The phylogenetic relationship between SFG rickettsiae were inferred from the comparison of both the gene and derived protein sequences, using the parsimony, neighbor-joining and maximum-likelihood methods. Three strongly supported phylogenetic sub-groups were distinguished: first, the Rickettsia conorii complex (R. conorii Malish, R. conorii M1, R. conorii Moroccan, R. conorii Indian tick typhus, Astrakhan fever rickettsia and Israeli tick typhus rickettsia); second, a cluster including Rickettsia africae, strain S, Rickettsia parkeri, Rickettsia sibirica and 'Rickettsia mongolotimonae'; and, third, a cluster including Rickettsia aeschlimannii, Rickettsia rhipicephali, Rickettsia massiliae, Bar 29 and Rickettsia montanensis. Rickettsia rickettsii, Rickettsia japonica, Rickettsia slovaca and Thai tick typhus rickettsia did not cluster with any other Rickettsia species. To test whether positive selection was responsible for sequences diversity, rates of synonymous and nonsynonymous nucleotide substitutions were compared for Rickettsia ompA alleles and indicated that this gene is undergoing neutral evolution.
Citations
Cited by
- Gene Sequence-Based Criteria for Identification of NewRickettsiaIsolates and Description ofRickettsia heilongjiangensissp. nov
- Current Knowledge on Phylogeny and Taxonomy of Rickettsia spp.
- First isolation of Rickettsia monacensis from a patient in South Korea
- Spotted fever group rickettsia closely related to Rickettsia monacensis isolated from ticks in South Jeolla province, Korea
- Acute tick-borne rickettsiosis caused by Rickettsia heilongjiangensis in Russian Far East. [europepmc]
- Rickettsia felis, Bartonella henselae, and B. clarridgeiae, New Zealand. [europepmc]
- Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii strains. [europepmc]
- Type V protein secretion pathway: the autotransporter story. [europepmc]
- Genetic differentiation of Chinese isolates of Rickettsia sibirica by partial ompA gene sequencing and multispacer typing. [europepmc]
- Japanese spotted fever, South Korea. [europepmc]
- RETRACTED: Tick-borne rickettsioses, neglected emerging diseases in rural Senegal. [europepmc]
- Characterization of Micrococcus strains isolated from indoor air. [europepmc]
- RETRACTED: Rickettsia species in African Anopheles mosquitoes. [europepmc]
- Rickettsia africae in Amblyomma variegatum ticks, Uganda and Nigeria. [europepmc]
- Detection of Rickettsia in Rhipicephalus sanguineus ticks and Ctenocephalides felis fleas from southeastern Tunisia by reverse line blot assay. [europepmc]
- Multispacer typing of Rickettsia isolates from humans and ticks in Tunisia revealing new genotypes. [europepmc]
- Detection of Rickettsia rickettsii, Rickettsia parkeri, and Rickettsia akari in skin biopsy specimens using a multiplex real-time polymerase chain reaction assay. [europepmc]
- Feeding by Amblyomma maculatum (Acari: Ixodidae) enhances Rickettsia parkeri (Rickettsiales: Rickettsiaceae) infection in the skin. [europepmc]
- First molecular detection of Rickettsia africae in ticks from the Union of the Comoros. [europepmc]
- High prevalence of Rickettsia africae variants in Amblyomma variegatum ticks from domestic mammals in rural western Kenya: implications for human health. [europepmc]
- Candidatus 'Rickettsia senegalensis' in cat fleas in Senegal. [europepmc]
- Evidence for a specific host-endosymbiont relationship between 'Rickettsia sp. genotype RF2125' and Ctenocephalides felis orientis infesting dogs in India. [europepmc]
- Francisella-Like Endosymbionts and Rickettsia Species in Local and Imported Hyalomma Ticks. [europepmc]
- Molecular detection of pathogens in ticks infesting cattle in Nampula province, Mozambique. [europepmc]
- Eco-epidemiological analysis of rickettsial seropositivity in rural areas of Colombia: A multilevel approach. [europepmc]
- A Concise Review of the Epidemiology and Diagnostics of Rickettsioses: Rickettsia and Orientia spp. [europepmc]
- Imported Hyalomma ticks in Germany in 2018. [europepmc]
- Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan. [europepmc]
- Dermacentor marginatus and Dermacentor reticulatus , and Their Infection by SFG Rickettsiae and Francisella -Like Endosymbionts, in Mountain and Periurban Habitats of Northwestern Italy. [europepmc]
Related