Deep amplicon sequencing as a powerful new tool to screen for sequence polymorphisms associated with anthelmintic resistance in parasitic nematode populations

2018/11/22 by Russell W. Avramenko, Elizabeth M. Redman, Elizabeth Redman +6 · 29 citations
Veterinary · Environmental Science · Immunology and Microbiology · #Helminth infection and control #Parasite Biology and Host Interactions #Parasites and Host Interactions

paper · doi:10.1016/j.ijpara.2018.10.005

Citations

• Diversity of benzimidazole-resistance alleles in populations of small ruminant parasites

Cited by

• A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp.
• Genomic landscape of drug response reveals mediators of anthelmintic resistance
• Challenges and opportunities for the adoption of molecular diagnostics for anthelmintic resistance
• A repeatable and quantitative DNA metabarcoding assay to characterize mixed strongyle infections in horses
• High Endemicity of Soil-Transmitted Helminths in a Population Frequently Exposed to Albendazole but No Evidence of Antiparasitic Resistance. [europepmc]
• Development of amplicon sequencing for the analysis of benzimidazole resistance allele frequencies in field populations of gastrointestinal nematodes. [europepmc]
• Evaluation of DNA Extraction Methods on Individual Helminth Egg and Larval Stages for Whole-Genome Sequencing. [europepmc]
• Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? [europepmc]
• New codon 198 β-tubulin polymorphisms in highly benzimidazole resistant Haemonchus contortus from goats in three different states in Sudan. [europepmc]
• Assessing anthelmintic resistance risk in the post-genomic era: a proof-of-concept study assessing the potential for widespread benzimidazole-resistant gastrointestinal nematodes in North American cattle and bison. [europepmc]
• Large scale screening for benzimidazole resistance mutations in Nematodirus battus, using both pyrosequence genotyping and deep amplicon sequencing, indicates the early emergence of resistance on UK sheep farms. [europepmc]
• Piloting a surveillance system to monitor the global patterns of drug efficacy and the emergence of anthelmintic resistance in soil-transmitted helminth control programs: a Starworms study protocol. [europepmc]
• Quantitative benzimidazole resistance and fitness effects of parasitic nematode beta-tubulin alleles. [europepmc]
• Teladorsagia circumcincta beta tubulin: the presence of the E198L polymorphism on its own is associated with benzimidazole resistance. [europepmc]
• Challenges and opportunities for the adoption of molecular diagnostics for anthelmintic resistance. [europepmc]
• Susceptible trichostrongyloid species mask presence of benzimidazole-resistant Haemonchus contortus in cattle. [europepmc]
• Exploring the β-tubulin gene family in a benzimidazole-resistant Parascaris univalens population. [europepmc]
• Multiple drug resistance in hookworms infecting greyhound dogs in the USA. [europepmc]
• Characterization of the β-tubulin gene family in Ascaris lumbricoides and Ascaris suum and its implication for the molecular detection of benzimidazole resistance. [europepmc]
• Newly identified parasitic nematode beta-tubulin alleles confer resistance to benzimidazoles. [europepmc]
• Regional heterogeneity and unexpectedly high abundance of Cooperia punctata in beef cattle at a northern latitude revealed by ITS-2 rDNA nemabiome metabarcoding. [europepmc]
• Understanding the role of wild ruminants in anthelmintic resistance in livestock. [europepmc]
• Absence of Polymorphisms in Codons 167, 198 and 200 of All Seven β-Tubulin Isotypes of Benzimidazole Susceptible and Resistant Parascaris spp. Specimens from Australia. [europepmc]
• Advances in diagnosis and control of anthelmintic resistant gastrointestinal helminths infecting ruminants. [europepmc]
• Benzimidazoles cause lethality by inhibiting the function of Caenorhabditis elegans neuronal beta-tubulin. [europepmc]
• Molecular evidence of widespread benzimidazole drug resistance in Ancylostoma caninum from domestic dogs throughout the USA and discovery of a novel β-tubulin benzimidazole resistance mutation. [europepmc]
• Emergence of Ancylostoma caninum parasites with the benzimidazole resistance F167Y polymorphism in the US dog population. [europepmc]
• Altered larval activation response associated with multidrug resistance in the canine hookworm Ancylostoma caninum . [europepmc]
• First Report of Benzimidazole Resistance in Field Population of Haemonchus contortus from Sheep, Goats and Cattle in Bosnia and Herzegovina. [europepmc]