2018/11/28 by Summer A. Baker Dockrey, Tyler J. Doyon, Jonathan C. Perkins +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · #Enzyme Catalysis and Immobilization #Microbial Metabolic Engineering and Bioproduction #Biochemical and biochemical processes
paper · pdf · doi:10.1111/cbdd.13443
openalex publication_date 2018/11/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
Technologies enabling new enzyme discovery and efficient protein engineering have spurred intense interest in the development of biocatalytic reactions. In recent years, whole-cell biocatalysis has received attention as a simple, efficient, and scalable biocatalytic reaction platform. Inspired by these developments, we have established a whole-cell protocol for oxidative dearomatization of phenols using the flavin-dependent monooxygenase, TropB. This approach provides a scalable biocatalytic platform for accessing gram-scale quantities of chiral synthetic building blocks.