2013/09/09 by Sang‐Wook Han, Malinee Sriariyanun, Sang‐Won Lee +4 · 1 citation
Biochemistry, Genetics and Molecular Biology · #Biochemical and Structural Characterization #Microbial Inactivation Methods #Bacterial biofilms and quorum sensing
paper · pdf · doi:10.1371/annotation/880a72e1-9cf3-45a9-bf1c-c74ccb73fd35
openalex publication_date 2013/09/09 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/14
The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axY S 22, derived from the Ax21 (activator of XA21-mediated immunity) protein. The conservation of Ax21 in all sequenced Xanthomonas spp. and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in Xanthomonas oryzae pv. oryzae. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the Xoo RaxH histidine kinase serves as the bacterial receptor for Ax21. This work establishes a critical role for small protein-mediated QS in a Gram-negative bacterium.