Concurrent knock‐out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae

1999/12/27 by Roman Wieczorke, Stefanie Krampe, Thomas Weierstall +3 · 4 citations
Biochemistry, Genetics and Molecular Biology · Materials Science · Agricultural and Biological Sciences · #Fungal and yeast genetics research #Enzyme Structure and Function #Fermentation and Sensory Analysis

paper · pdf · doi:10.1016/s0014-5793(99)01698-1

openalex publication_date 1999/12/27 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/25

Abstract

The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport hexoses. In a yeast strain deleted for HXT1-17, GAL2, AGT1, YDL247w and YJR160c, glucose consumption and transport activity were completely abolished. However, as additional deletion of the glucose sensor gene SNF3 partially restored growth on hexoses, our data indicate the existence of even more proteins able to transport hexoses in yeast.

Citations

• An efficient transformation procedure enabling long‐term storage of competent cells of various yeast genera
• Investigation of two yeast genes encoding putative isoenzymes of phosphoglycerate mutase

Cited by

• The hemibiotrophic lifestyle of Colletotrichum species
• Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network
• GLUT14, a Duplicon of GLUT3, Is Specifically Expressed in Testis as Alternative Splice Forms
• Eckhard Boles [wikipedia]