2021/10/01 by Margarita Darder, Jing He, Laurent Charlet +4 · 13 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Health Professions · Materials Science · #Adsorption #Bacteria #Chemistry #Clay minerals and soil interactions #Hydrogels: synthesis, properties, applications #Intercalation (chemistry) #Materials science #Montmorillonite #Nuclear chemistry #Organic chemistry #Shewanella putrefaciens #Therapeutic Uses of Natural Elements #Thermogravimetric analysis
paper · pdf · doi:10.1007/s42860-021-00156-3
published in Clays and Clay Minerals 69(5), 576-588 (Cambridge University Press)
crossref issued 2021/10/01 · crossref published 2021/10/01 · crossref published-print 2021/10/01 · openalex publication_date 2021/10/01 · crossref created 2022/01/07 · crossref published-online 2024/01/01 · crossref deposited 2024/03/11 · openalex created_date 2025/10/10 · crossref indexed 2026/08/03 · openalex updated_date 2026/08/04
Abstract The present study introduces an overview of gentamicin-clay mineral systems for applications in biomedicine and then focuses on the development of a series of gentamicin/clay hybrid materials to be used as the bioactive phase of hydroxypropylmethylcellulose (HPMC) to produce bionanocomposite membranes possessing antimicrobial activity of interest in wound-dressing applications. Gentamicin (Gt) was adsorbed from aqueous solutions into a montmorillonite (Cloisite®-Na + ) to produce intercalation compounds with tunable content of the antibiotic. The hybrids were characterized by CHN chemical analysis, energy-dispersive X-ray analysis, X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis, confirming the intercalation of Gt by an ion-exchange mechanism. The release of Gt from the hybrids was tested in water and in buffer solution to check their stability. Hybrids with various amounts of Gt were incorporated into a HPMC matrix at various loadings and processed as films by the casting method. The resulting Gt-clay/HPMC bionanocomposites were characterized by means of field-emission scanning electron microscopy, and were also evaluated for their water-adsorption and mechanical properties to confirm their suitability for wound-dressing applications. The antimicrobial activity of the bionanocomposite films was tested in vitro toward various microorganisms ( Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) , vancomycin-resistant Enterococcus faecium, Acinetobacter baumannii , and Klebsiella pneumonia ), showing a complete bacterial reduction even in films with small Gt contents.