2013/05/21 by Dagmar Procházková, D. Prochazkova, N. Wilhelmová +6 · 10 citations
Agricultural and Biological Sciences · Chemistry · #Aluminum toxicity and tolerance in plants and animals #Biochemistry #Biology #Botany #Chemistry #Comet assay #DNA #DNA damage #Gel electrophoresis #Gene #Horticulture #Molecular biology #Nicotiana #Nicotiana tabacum #Plant Micronutrient Interactions and Effects #Plant Stress Responses and Tolerance #Shoot #Solanaceae #Somatic cell #Zinc
paper · pdf · doi:10.1007/s10535-013-0345-x
published in Biologia Plantarum 57(4), 783-787 (Institute of Experimental Botany of the Czech Academy of Sciences)
openalex publication_date 2013/05/21 · crossref created 2013/05/21 · crossref issued 2013/12/01 · crossref published 2013/12/01 · crossref published-print 2013/12/01 · crossref deposited 2020/06/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31 · crossref indexed 2026/08/04
We applied the alkaline version of the single-cell gel electrophoresis (comet assay) to seedlings of heterozygous tobacco (Nicotiana tabacum L. var. xanthi) treated with zinc acetate dihydrate (20 to 80 mM Zn2+ for 2 h or 2 to 12 mM Zn2+ for 24 h). A dose dependent increase in DNA damage expressed by the tail moment values were observed in nuclei isolated from the roots after 2 and 24 h Zn2+ treatments. In contrast, Zn2+ did not induce significant DNA damage to leaf nuclei, with the exception of 10 or 12 mM Zn2+ for 24 h. Somatic mutations, identified as dark green, yellow, and dark green/yellow double sectors on the pale green tobacco leaves were not detected after any Zn2+ treatments. The accumulation of Zn in roots and shoots was determined by inductively coupled plasma optical emission spectrometry and the Zn content in roots was about three times higher than in shoots.