Capsular Polysaccharide Is a Major Complement Resistance Factor in Lipopolysaccharide O Side Chain-Deficient <i>Klebsiella pneumoniae</i> Clinical Isolates

2000/02/01 by Dolores Vargas Álvarez, Dolores Álvarez, Susana Merino +6 · 28 citations
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · Medicine · #Antibiotic Resistance in Bacteria #Complement system in diseases #Pneumonia and Respiratory Infections

paper · doi:10.1128/iai.68.2.953-955.2000

Abstract

We have previously demonstrated the existence of Klebsiella pneumoniae clinical isolates deficient in the lipopolysaccharide O side chain, the major factor for resistance to complement-mediated killing in this bacterial species. These isolates are complement resistant, and their mechanisms to resist complement were investigated by selecting transposon-generated complement-sensitive mutants. One mutant with a drastically reduced capacity to grow in nonimmune human serum carried the transposon inserted in an open reading frame of a gene cluster involved in capsule synthesis. This mutant produced less capsule, bound more molecules of the complement component C3, and was more sensitive to complement-mediated and opsonophagocytic killings than was the parent strain. Four additional clinical isolates representing four different K serotypes were studied, and results showed that capsular polysaccharide is a major complement resistance factor in these O side chain-deficient isolates.

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