Chemical and immunological characterization of the extracellular galactomannan of Aspergillus fumigatus
1994/12/01 by J P Latgé, Jean‐Paul Latgé, Hidemitsu Kobayashi +14 · 30 citations
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Glycosylation and Glycoproteins Research #Enzyme Production and Characterization #Polysaccharides and Plant Cell Walls
paper · doi:10.1128/iai.62.12.5424-5433.1994
Abstract
The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate. Nuclear magnetic resonance and gas-liquid chromatography-mass spectrometry analysis have been performed on intact GM, acid-hydrolyzed GM, and oligomers resulting from the acetolysis of the acid-hydrolyzed GM. Results show that A. fumigatus GM is composed of a linear mannan core with an alpha-(1-2)-linked mannotetraose repeating unit attached via alpha-(1-6) linkage. Side chains composed of an average of 4 to 5 beta-(1-5)-galactofuranose units are linked to C-6 and C-3 positions of alpha-(1-2)-linked mannose units of the mannan. The immunoreactivity of GM and HCl-hydrolyzed GM was studied by use of human sera from aspergillosis patients and an antigalactofuran monoclonal antibody. The alpha-(1-2) (1-6)-mannan core is not antigenic. The immunogenic galactofuran is found amongst several exocellular glycoproteins. According to a direct enzyme-linked immunosorbent assay with GM as the detector antigen, only 26% of the serum samples from aspergilloma patients (all positive by immunodiffusion assays) give optical density values superior to a cutoff estimated as the mean +/- 3 standard deviations of values obtained with control sera.
Citations
Cited by
- Galactosaminogalactan ofAspergillus fumigatus, a bioactive fungal polymer
- The Cell Wall of the Human Fungal Pathogen Aspergillus fumigatus: Biosynthesis, Organization, Immune Response, and Virulence
- Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions
- The structure and synthesis of the fungal cell wall
- Mannosylation in Candida albicans: role in cell wall function and immune recognition
- Reactivity of platelia Aspergillus galactomannan antigen with piperacillin-tazobactam: clinical implications based on achievable concentrations in serum. [europepmc]
- Aspergillus infections in transplant recipients. [europepmc]
- Galactomannoproteins of Aspergillus fumigatus. [europepmc]
- Nippostrongylus brasiliensis: identification of intelectin-1 and -2 as Stat6-dependent genes expressed in lung and intestine during infection. [europepmc]
- Ligand binding and substrate discrimination by UDP-galactopyranose mutase. [europepmc]
- Molecular mechanism of Aspergillus fumigatus adherence to host constituents. [europepmc]
- GfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and Aspergillus fumigatus. [europepmc]
- Galactomannan detection for invasive aspergillosis in immunocompromised patients. [europepmc]
- Insights into furanose solution conformations: beyond the two-state model. [europepmc]
- Novel mouse monoclonal antibodies specifically recognize Aspergillus fumigatus galactomannan. [europepmc]
- Urine Antigen Detection as an Aid to Diagnose Invasive Aspergillosis. [europepmc]
- Identification of Two Mannosyltransferases Contributing to Biosynthesis of the Fungal-type Galactomannan α-Core-Mannan Structure in Aspergillus fumigatus. [europepmc]
- Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus . [europepmc]
- Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan. [europepmc]
- Targeting the fungal cell wall: current therapies and implications for development of alternative antifungal agents. [europepmc]
- Galactomannan detection in broncho-alveolar lavage fluid for invasive aspergillosis in immunocompromised patients. [europepmc]
- The Glycosylphosphatidylinositol-Anchored DFG Family Is Essential for the Insertion of Galactomannan into the β-(1,3)-Glucan-Chitin Core of the Cell Wall of Aspergillus fumigatus. [europepmc]
- Aspergillus fumigatus and Aspergillosis in 2019. [europepmc]
- Potential of Chemically Synthesized Oligosaccharides To Define the Carbohydrate Moieties of the Fungal Cell Wall Responsible for the Human Immune Response, Using Aspergillus fumigatus Galactomannan as a Model. [europepmc]
- Galactomannan Produced by Aspergillus fumigatus : An Update on the Structure, Biosynthesis and Biological Functions of an Emblematic Fungal Biomarker. [europepmc]
- LYSMD3: A mammalian pattern recognition receptor for chitin. [europepmc]
- Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing. [europepmc]
- A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR. [europepmc]
- Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants. [europepmc]
- The C-Type Lectin Receptor Dectin-2 Is a Receptor for Aspergillus fumigatus Galactomannan. [europepmc]
Related