Staphylococcus aureus Peptidoglycan Is a Toll-Like Receptor 2 Activator: a Reevaluation

2005/07/22 by Roman Dziarski, Dipika Gupta · 25 citations
Immunology and Microbiology · Health Professions · #Immune Response and Inflammation #Antimicrobial Peptides and Activities #Pediatric health and respiratory diseases

paper · doi:10.1128/iai.73.8.5212-5216.2005

Abstract

Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 mug/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.

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