2022/05/16 by Ryan Wallis, Deborah Milligan, Bethany K. Hughes +6 · 1 voice
Medicine · Biochemistry, Genetics and Molecular Biology · #Telomeres, Telomerase, and Senescence #Cell Image Analysis Techniques #Genetics, Aging, and Longevity in Model Organisms
paper · pdf · doi:10.18632/aging.204072
openalex publication_date 2022/05/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Senescence occurs in response to a number of damaging stimuli to limit oncogenic transformation and cancer development. As no single, universal senescence marker has been discovered, the confident classification of senescence induction requires the parallel assessment of a series of hallmarks. Therefore, there is a growing need for âfirst-passâ tools of senescence identification to streamline experimental workflows and complement conventional markers. Here, we utilise a high content, multidimensional phenotypic profiling-based approach, to assess the morphological profiles of senescent cells induced via a range of stimuli. In the context of senescence, we refer to these as senescence-associated morphological profiles (SAMPs), as they facilitate distinction between senescent and proliferating cells. The complexity of the profiles generated also allows exploration of the heterogeneity both between models of senescence and within an individual senescence model, providing a level of insight at the single cell level. Furthermore, we also demonstrate that these models are applicable to the assessment of senescence in vivo, which remains a key challenge for the field. Therefore, we believe SAMPs has the potential to serve as a useful addition in the repertoire of senescence researchers, either as a first-pass tool or as part of the established senescence hallmarks.