2018/12/05 by Andrew Price, Anna‐Lena Cost, Hanna Ungewiß +3 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Cellular Mechanics and Interactions #Skin and Cellular Biology Research #Cardiomyopathy and Myosin Studies
paper · pdf · doi:10.1038/s41467-018-07523-0
openalex publication_date 2018/12/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
Desmosomes are intercellular adhesion complexes that connect the intermediate filament cytoskeletons of neighboring cells, and are essential for the mechanical integrity of mammalian tissues. Mutations in desmosomal proteins cause severe human pathologies including epithelial blistering and heart muscle dysfunction. However, direct evidence for their load-bearing nature is lacking. Here we develop Förster resonance energy transfer (FRET)-based tension sensors to measure the forces experienced by desmoplakin, an obligate desmosomal protein that links the desmosomal plaque to intermediate filaments. Our experiments reveal that desmoplakin does not experience significant tension under most conditions, but instead becomes mechanically loaded when cells are exposed to external mechanical stresses. Stress-induced loading of desmoplakin is transient and sensitive to the magnitude and orientation of the applied tissue deformation, consistent with a stress absorbing function for desmosomes that is distinct from previously analyzed cell adhesion complexes.