2024/07/16 by Emmanuel Soubies, Alejandro Nogueron, Florence Pelletier +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Optical Coherence Tomography Applications #Digital Holography and Microscopy
paper · doi:10.1111/jmi.13344
openalex publication_date 2024/07/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Super-resolution structured-illumination microscopy (SIM) is a powerful technique that allows one to surpass the diffraction limit by up to a factor two. Yet, its practical use is hampered by its sensitivity to imaging conditions which makes it prone to reconstruction artefacts. In this work, we present FlexSIM, a flexible SIM reconstruction method capable to handle highly challenging data. Specifically, we demonstrate the ability of FlexSIM to deal with the distortion of patterns, the high level of noise encountered in live imaging, as well as out-of-focus fluorescence. Moreover, we show that FlexSIM achieves state-of-the-art performance over a variety of open SIM datasets.