2018/02/01 by Aldrik H. Velders, C.D. Schoen, Vittorio Saggiomo · 1 voice
Engineering · Biochemistry, Genetics and Molecular Biology · #Biosensors and Analytical Detection #Advanced Chemical Sensor Technologies #Spectroscopy Techniques in Biomedical and Chemical Research
paper · pdf · doi:10.1186/s13104-018-3197-9
openalex publication_date 2018/02/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. RESULTS: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field.