2017/06/28 by Nhan Lu-Chinh Phan, Jenny J. Hong, Bobby Tofig +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · Medicine · #3D Printing in Biomedical Research #Cancer Cells and Metastasis #Cellular Mechanics and Interactions
paper · pdf · doi:10.1101/138412
openalex publication_date 2017/06/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Abstract There is increasing interest in developing 3D tumor organoid models for drug development and personalized medicine applications. While tumor organoids are in principle amenable to high-throughput drug screenings, progress has been hampered by technical constraints and extensive manipulations required by current methodologies. Here, we introduce a miniaturized, fully automatable, flexible high-throughput method using a simplified geometry to rapidly establish 3D organoids from cell lines and primary tissue and robustly assay drug responses. As proof of principle, we use our miniring approach to establish organoids of high-grade serous tumors and one carcinosarcoma of the ovaries and screen hundreds of protein kinase compounds currently FDA-approved or in clinical development. In all cases we could identify drugs causing significant reduction in cell viability, number and size of organoids within a week from surgery, a timeline compatible with therapeutic decision making.