2024/10/23 by Ling Wang, Mengyu Zhao, Xue Zhang +5 · 1 voice · 13 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Biology #Biotechnology #Botany #Cell biology #Cold tolerance #DNA #DNA ligase #Gene #Genetics #Horticultural and Viticultural Research #Horticulture #Plant Gene Expression Analysis #Plant Stress Responses and Tolerance #Ubiquitin #Ubiquitin ligase
paper · doi:10.1093/hr/uhae297
published in Horticulture Research 12(2), uhae297 (Nature Portfolio)
openalex publication_date 2024/10/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/25
Abstract Cold stress seriously affects plant growth and development. The ubiquitination system plays an important role by degrading and modifying substrates at the protein level. In this study, the U-box type ubiquitin ligase VviPUB19 gene was induced by low temperature (4°C) in grapevine. In Arabidopsis thaliana, the pub19 mutant, a homologous mutation of VviPUB19, exhibited enhanced cold tolerance, and the resistance phenotype of the mutant could be attenuated by VviPUB19. VviPUB19-overexpressing grape lines exhibited lower cold tolerance. Furthermore, it was revealed that VviPUB19 interacted with the cold-related transcription factors VviICE1, 2, and 3 and VviCBF1 and 2, and was involved in the degradation of them. This is the first time that an E3 ligase (VviPUB19) that interacts with CBFs and affects its protein stability has been identified. It was also shown that VviICE1, 2, and 3 positively regulated VviPUB19 promoter activity. Therefore, our results suggest that VviPUB19 reduces grape cold tolerance via participating in the CBF-dependent pathway.