2021/06/23 by Alessio Sacco, Chiara Portesi, Andrea Mario Giovannozzi +1 · 1 voice
Biochemistry, Genetics and Molecular Biology · Materials Science · #Spectroscopy Techniques in Biomedical and Chemical Research #Gold and Silver Nanoparticles Synthesis and Applications #Advanced Fluorescence Microscopy Techniques
paper · pdf · doi:10.1002/jrs.6187
openalex publication_date 2021/06/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/03
Abstract In this work, a layer of graphene was used as a standard material for the measurement of the dimensions of Raman microscopes focal volumes of different confocal Raman spectrometers equipped with different objectives and excitation laser wavelengths. This method consists in probing the volume near the focal point of the system by using a flat graphene monolayer sheet with a straight edge. Graphene was selected because of its high Raman cross section and mechanically and chemically stability, allowing fast measurements and easy manipulation. In this paper, a method to employ graphene to accurately and precisely measure the three dimensions of the focal volume of a Raman microscope is presented; scanning along the axial and lateral directions, it is possible to reconstruct the three dimensions of the focal volume. Furthermore, these operations can be combined in a single procedure which allows the measurement of projections of the volume on planes parallel to the optical axis. Knowledge of these parameters enable absolute quantification of Raman‐active molecules and support high‐resolution Raman imaging.