2025/03/14 by Sheena Dass, Prasun Kundu, Deboki Naskar +4 · 1 voice
Medicine · Immunology and Microbiology · #Malaria Research and Control #Vector-borne infectious diseases #Complement system in diseases
paper · doi:10.1093/infdis/jiaf136
openalex publication_date 2025/03/14 · openalex created_date 2025/03/15 · openalex updated_date 2026/08/01
Plasmodium vivax vaccine discovery lags behind Plasmodium falciparum due to the absence of a reliable in vitro culture system. We developed a miniature 384-well assay for the evolutionarily related parasite species Plasmodium cynomolgi and Plasmodium knowlesi, deploying it for screening invasion inhibition efficacy of antibodies elicited against 8 P. vivax proteins. All 8 antibodies showed consistent inhibition across both P. knowlesi and P. cynomolgi species, correlating with inhibition in ex vivo P. vivax isolates. The use of simian malaria parasites and this assay represent a robust, high-throughput method for prioritizing P. vivax blood-stage vaccine candidates.